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Formalin-fixed and paraffin-embedded rat colon labeled with Rabbit Anti-CD163\/M130 Polyclonal Antibody, Unconjugated( bs-2527R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163\/M130 Polyclonal Antibody, Unconjugated (bs-2527R) followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse uterus lysates probed (bs-2527R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 130kDa.
Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163\/M130 Polyclonal Antibody, unconjugated (bs-2527R) at 1:100 for 40 minutes at 37\u00b0C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37\u00b0C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative. \\n

Catalog #1: bs-2527R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Unconjugated

U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163\/M130 Polyclonal Antibody,FITC Conjugated(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2527R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: FITC

Catalog #1: bs-2527R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: PE

Catalog #1: bs-2527R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy5

Catalog #1: bs-2527R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy3

Catalog #1: bs-2527R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Biotin

Catalog #1: bs-2527R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy7

Catalog #1: bs-2527R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: HRP

Catalog #1: bs-2527R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: Cy5.5

Catalog #1: bs-2527R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 350

Catalog #1: bs-2527R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 405

Catalog #1: bs-2527R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 488

Catalog #1: bs-2527R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 555

Catalog #1: bs-2527R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 594

Catalog #1: bs-2527R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 647

Catalog #1: bs-2527R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 680

Catalog #1: bs-2527R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: AbBy Fluor® 750

Catalog #1: bs-2527R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: APC

Catalog #1: bs-2527R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: APC-Cy5.5

Catalog #1: bs-2527R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: APC-Cy7

Catalog #1: bs-2527R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: PE-Cy5

Catalog #1: bs-2527R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: PE-Cy5.5

Catalog #1: bs-2527R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: PE-Cy7

Catalog #1: bs-2527R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: PerCP

Catalog #1: bs-2527R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2527R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Others

Conjugate: RBITC