ROCK1 (3A1) Monoclonal Antibody

$Lane 1: A431 cells; Lane 2: NIH\/3T3 cells; Probed with ROCK1 (3A1) Monoclonal Antibody (bsm-52470R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A431 cells; Lane 2: NIH\/3T3 cells; Probed with ROCK1 (3A1) Monoclonal Antibody (bsm-52470R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ROCK1 (3A1) Monoclonal Antibody (bsm-52470R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ROCK1 (3A1) Monoclonal Antibody (bsm-52470R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52470R
Product Name	ROCK1 (3A1) Monoclonal Antibody
Applications	WB, FCM, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human ROCK1 between 1100-1200 amino acids
Clonality	Monoclonal
Clone #	3A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6093
Swiss Prot	Q13464
Synonyms	Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, p160 ROCK-1, ROCK-I, p160ROCK, ROCK1
Background	The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IHC