AKT1S1 Monoclonal Antibody

$Lane 1: 293 cell lysates; Lane 2: Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with AKT1S1 Monoclonal Antibody, Unconjugated (bsm-51706M) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 293 cell lysates; Lane 2: Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with AKT1S1 Monoclonal Antibody, Unconjugated (bsm-51706M) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bsm-51706M
Product Name	AKT1S1 Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human AKT1S1.
Clonality	Monoclonal
Clone #	F6F22
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84335
Swiss Prot	Q96B36
Subcellular location	Cytoplasm
Synonyms	PRAS40; AKT1 substrate 1 (proline rich); AKT1 substrate 1; AKT1-S1; AKT1S-1; AKT1S 1; AKT1 S1; Lobe; PRAS 40; PRAS-40; Proline rich akt substrate; 40 kDa proline rich AKT substrate; 40 kDa proline-rich AKT substrate; AKT1 substrate 1; AKTS1_HUMAN; MGC2865; Proline rich Akt substrate 40 kDa; Proline-rich AKT1 substrate 1.
Background	Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Application Dilution
WB	=1:1000-2000Ôºå