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ROCK1 Polyclonal Antibody

Applications

  • WB
  • IHC-P
  • IP
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-55188R
Product Name ROCK1 Polyclonal Antibody
Applications WB, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 6093
Swiss Prot Q13464
Subcellular location Cytoplasm, Cell membrane
Synonyms ROCK1;P160ROCK;ROCK-I
Background This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IP 1-2ug
IHC