NG/Neurogranin Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Neurogranin Polyclonal Antibody, Unconjugated(bs-11435R) 1:200, followed by conjugation to the secondary antibody and DAB.

$Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat brian, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat liver, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (NG\/Neurogranin) at 37\u00b0C for 30min; Antibody incubation with (HDAC4) Polyclonal Antibody, Unconjugated (bs-11435R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.$

Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat brian, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (rat liver, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (NG\/Neurogranin) at 37\u00b0C for 30min; Antibody incubation with (HDAC4) Polyclonal Antibody, Unconjugated (bs-11435R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.

$Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (mouse brain, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (mouse heart, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (NG\/Neurogranin) at 37\u00b0C for 30min; Antibody incubation with (HDAC4) Polyclonal Antibody, Unconjugated (bs-11435R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.$

Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (mouse brain, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (mouse heart, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (NG\/Neurogranin) at 37\u00b0C for 30min; Antibody incubation with (HDAC4) Polyclonal Antibody, Unconjugated (bs-11435R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-11435R
Product Name	NG/Neurogranin Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NG/Neurogranin
Immunogen Range	30-78/78
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4900
Swiss Prot	Q92686
Subcellular location	Cytoplasm
Synonyms	RC3; hng; Neurogranin; Ng; NRGN
Background	Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500