EZH2 (1D12) Monoclonal Antibody

$ChIP assays were performed using 2.5 \u03bcg of EZH2 (1D12) Monoclonal Antibody (bsm-53018M) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-\/- cells (negative control). IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and T gene which encodes the Brachyury transcription factor. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).$

ChIP assays were performed using 2.5 \u03bcg of EZH2 (1D12) Monoclonal Antibody (bsm-53018M) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-\/- cells (negative control). IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and T gene which encodes the Brachyury transcription factor. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-53018M
Product Name	EZH2 (1D12) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Monoclonal antibody raised in mouse against the central part of the human EZH2 protein (Enhancer of zeste homolog 2).
Clonality	Monoclonal
Clone #	1D12
Isotype	IgG1
Concentration	1ug/ul
Purification	Protein G purified polyclonal antibody in PBS containing 0.05% azide.
Storage Buffer	PBS with 0.05% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2146
Swiss Prot	Q15910
Synonyms	Histone-lysine N-methyltransferase EZH2, EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, KMT6
Background	Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Application Dilution
WB	1:300-5000