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JMJD2a Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IF

Reactivity

  • Human
Overview
Catalog # bs-53136R
Product Name JMJD2a Polyclonal Antibody
Applications WB, ELISA, IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein
Clonality Polyclonal
Isotype Not Determined
Concentration 0.6ug/ul
Purification Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Storage Buffer PBS with 0.05% sodium azide and 0.05% Proclin300.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 9682
Swiss Prot O75164
Synonyms JHDM3A, KDM4A, JMJD2, Lysine-specific demethylase 4A
Background Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IF