PAPD5 Polyclonal Antibody

$Nuclear extracts of HeLa cells (40 \u03bcg) were analyzed by Western blot using the antibody against PAPD5 (bs-53073R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 63 kDa); the marker (in kDa) is shown on the left.$

Nuclear extracts of HeLa cells (40 \u03bcg) were analyzed by Western blot using the antibody against PAPD5 (bs-53073R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 63 kDa); the marker (in kDa) is shown on the left.

Applications

WB
ELISA

Reactivity

Human

Overview
Catalog #	bs-53073R
Product Name	PAPD5 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Polyclonal antibody raised in rabbit against human PAPD5 (PAP associated domain containing 5), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein
Clonality	Polyclonal
Isotype	Not Determined
Concentration	Not Determined
Purification	Whole antiserum from rabbit containing 0.05% azide
Storage Buffer	Serum with 0.05% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	64282
Swiss Prot	Q8NDF8
Synonyms	Non-canonical poly(A) RNA polymerase PAPD5, PAPD5, TRF4-2, TUTase 3, PAP-associated domain-containing protein 5
Background	Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000