SULT2A1 (3A1) Monoclonal Antibody

$Lane 1: Mouse Kidney; Probed with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Kidney; Probed with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Adrenal Gland tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Adrenal Gland tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Liver tissue incubated with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of HepG2 cells with SULT2A1 (3A1) Monoclonal Antibody (bsm-54362R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54362R
Product Name	SULT2A1 (3A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human SULT2A1 aa 100 to the C-terminus.
Clonality	Monoclonal
Clone #	3A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6822
Swiss Prot	Q06520
Subcellular location	Cytoplasm
Synonyms	Bile salt sulfotransferase; Dehydroepiandrosterone sulfotransferase; Hydroxysteroid Sulfotransferase; ST2A3; Sulfotransferase 2A1; DHEA-ST; HST; ST2A1; SULT2A1; STD
Background	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200