ACTH (18-39) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ACTH (18-39) Polyclonal Antibody, Unconjugated (bs-0442R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ACTH (18-39)Polyclonal Antibody, Unconjugated(bs-0442R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ACTH (18-39)Polyclonal Antibody, Unconjugated(bs-0442R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human

Overview
Catalog #	bs-0442R
Product Name	ACTH (18-39) Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ACTH
Immunogen Range	18-39/39
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5443
Swiss Prot	P01189
Subcellular location	Extracellular matrix
Synonyms	LPH; MSH; NPP; POC; ACTH; CLIP; Pro-opiomelanocortin; POMC; Corticotropin-lipotropin
Background	ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200