Due to the possibility of mismatching between antigens from other origin and antibodies used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
Principle of the Assay
| The kit uses enzyme-linked immunoassay. According to the principle of antibody blocking; the following design was made: The recombinant protein of SARS-CoV-2-N was used as a coating antigen and was coated with a 96-well reaction plate. When the positive sample is added and within a certain reaction time; the (solid-phase antigen-antibody) complex is formed. Wash the plate; get rid of the substance that didn't participate in the reaction. HRP anti-SARS-CoV-2-N antibody was added as a tracer. Antigenic reaction with no binding antibody on the reaction plate; Formation of (Solid-phase antigen - HRP antibody) complexes. Absorbance was measured 450nm using a microplate reader. The concentration of specific total antibody in the sample was inversely proportional to the absorbance (OD value). |
Target Information
| Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by infection with the SARS-CoV-2 virus. Common signs of infection include respiratory symptoms, fever, cough, shortness of breath and breathing difficulties. In severe cases, the infection can cause pneumonia, severe acute respiratory syndrome (SARS), kidney failure and death. Coronaviruses (CoV) are a large family of viruses that cause illness ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). SARS-CoV-2 is a new strain of coronavirus that was first identified in December 2019 when the first outbreak occurred in Wuhan, China. |
Materials Supplied
| Kit Components |
Microplate coated with SARS-CoV-2-N recombinant protein antigen. (8×12)
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Negative reference product; Specification: 0.8ml / bottle
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Positive reference product; Specification: 0.8ml / bottle
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Enzyme Conjugate: anti-SARS-CoV-2-N monoclonal antibody with HRP marker: Specification: 10.5ml / bottle.
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| Color rendering solution A, mainly composed of H2O2; Specification: 5.0ml / bottle. |
Color rendering solution B, mainly composed of which is TMB. Specification: 5.0ml / bottle.
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| Termination solution, mainly containing dilute H2SO4. Specification: 5.0ml / bottle. |
Concentrated washing solution, 10 times concentrated washing solution, diluted before use. Specification: 50.0ml / bottle.
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Operating instruction
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Storage
Performance Characteristics
| Tested the positive reference products of the enterprise. Repeated detection 10 times, all the results were positive. |
Review the datasheet for extended details.
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Data Analysis Assistance
We have partnered with MyAssays to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by MyAssays specifically for bskv0003.
https://www.myassays.com/bioss-sars-cov-2-total-antibody-elisa-kit.assay
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