AHSG Polyclonal Antibody

$Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti AHSG\/Fetuin A\/Alpha 2 HS Glycoprotein Polyclonal Antibody, Unconjugated (bs-2922R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 31kD. Observed band size: 31kD.$

Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti AHSG\/Fetuin A\/Alpha 2 HS Glycoprotein Polyclonal Antibody, Unconjugated (bs-2922R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 31kD. Observed band size: 31kD.

$Antigen: bs-2922P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-2922P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Lane 1: Mouse Liver lysates probed with AHSG Polyclonal Antibody, Unconjugated (bs-2922R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P

Reactivity

Mouse
Others

Predicted Reactivity

Human
Rat
Dog
Cow
Sheep
Horse
Rabbit

Overview
Catalog #	bs-2922R
Product Name	AHSG Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Mouse, Others
Predicted Reactivity	Human, Rat, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Alpha-2-HS-glycoprotein chain A.
Immunogen Range	201-300/367
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	197
Swiss Prot	P02765
Subcellular location	Secreted
Synonyms	AHS; A2HS; HSGA; FETUA; Alpha-2-HS-glycoprotein; Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-A; AHSG; PRO2743
Background	Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400