ZNT8 Polyclonal Antibody

Lane 1: Human Raji lysates; Lane 2: Mouse spleen lysates probed with Rabbit Anti-ZNT8 Polyclonal Antibody, Unconjugated (bs-15382R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with ZNT8 Polyclonal Antibody (bs-15382R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with ZNT8 Polyclonal Antibody (bs-15382R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Sheep
Pig

Overview
Catalog #	bs-15382R
Product Name	ZNT8 Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ZNT8
Immunogen Range	101-200/369
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	169026
Swiss Prot	Q8IWU4
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ZNT8; ZnT-8; Zinc transporter 8; Solute carrier family 30 member 8; SLC30A8
Background	Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400