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Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0014R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody (bs-0227R ) at 1:100 for 30 minutes followed by incubation with Anti-Rabbit IgG FITC conjugated secondary for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R Antibody(bs-0227R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R Polyclonal Antibody(bs-0227R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0227R

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-0014R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0014R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-0014R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-0227R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0227R-FITC

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0014R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0014R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-0014R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-0227R-Cy3

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0227R-Cy5

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-0227R-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-0227R-Cy5.5

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-0014R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-0227R-BF350

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0227R-BF405

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0227R-BF488

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0227R-BF555

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0227R-BF594

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0227R-BF647

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0227R-BF680

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0227R-BF750

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0227R-APC

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC

Catalog #1: bs-0227R-APC-Cy5.5

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy5.5

Catalog #1: bs-0227R-APC-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy7

Catalog #1: bs-0227R-PE-Cy5

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5

Catalog #1: bs-0227R-PE-Cy5.5

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5.5

Catalog #1: bs-0227R-PE-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy7

Catalog #1: bs-0227R-PerCP

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP

Catalog #1: bs-0227R-PerCP-Cy5.5

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0227R-RBITC

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: RBITC

Catalog #1: bs-0014R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0014R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0014R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0014R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0014R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0014R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0014R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0014R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0014R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC

Catalog #1: bs-0014R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy5.5

Catalog #1: bs-0014R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy7

Catalog #1: bs-0014R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP

Catalog #1: bs-0014R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0014R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: RBITC

Catalog #1: bs-0014R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0014R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5