COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody

$Flow cytometric analysis of HeLa cells with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of HeLa cells with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: C2C12 Cells; Lane 2: A431 Cells; Probed with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: C2C12 Cells; Lane 2: A431 Cells; Probed with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in C2C12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in C2C12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in L6 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in L6 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52569R
Product Name	COX1/Cyclooxygenase 1 (4A8) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human COX1/ Cyclooxygenase 1 between 400 to 600 amino acids
Clonality	Monoclonal
Clone #	4A8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5742
Swiss Prot	P23219
Synonyms	Prostaglandin G/H synthase 1, Cyclooxygenase-1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, COX-1, PGH synthase 1, PGHS-1, PHS 1, PTGS1
Background	Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC