| Overview |
| bs-8375R-Biotin |
| UBE2A Polyclonal Antibody, Biotin Conjugated |
| ELISA, IHC-P, IHC-F |
| Human, Mouse, Rat, Cow, Chicken |
| Specifications |
| Biotin |
| Rabbit |
| KLH conjugated synthetic peptide derived from human UBE2A/RAD6 |
| 81-152/152 |
| Polyclonal |
| IgG |
| 1ug/ul |
| Purified by Protein A. |
| Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Store at -20°C for 12 months. |
| Target |
| 7319 |
| Cytoplasm, Nucleus |
| BHR6A; hHR6A; HR6A; mHR6A; RAD6 homolog A; RAD6A; RAD6B; UBC-1; UBC2; UBC6; UBCD6; UBE2A; UBE2A_HUMAN; UBE2B; Ubiquitin carrier protein A; Ubiquitin carrier protein; Ubiquitin conjugating enzyme E2 17 kDa; Ubiquitin conjugating enzyme E2 21.5 kDa; Ubiquitin conjugating enzyme E2 A; Ubiquitin protein ligase A; Ubiquitin-conjugating enzyme E2 A; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-conjugating enzyme E2-21.5 kDa; Ubiquitin-protein ligase A. |
| The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (Ubiquitin-conjugating enzyme E2 A) and UBE2B (Ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to postreplication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18. |
| Application Dilution |
| ELISA |
1:500-1000 |
| IHC-P |
1:200-400 |
| IHC-F |
1:100-500 |
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