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TGF Beta 1+2+3 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Sheep
  • Rabbit

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Guinea Pig
Overview
Catalog # bs-4538R-APC
Product Name TGF Beta 1+2+3 Polyclonal Antibody, APC Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity This antibody will preferentially react with TGFB1, but will also detect TGFB2 (93%) and TGFB3 (86%) based upon sequence similarity.
Reactivity Human, Mouse, Rat, Sheep, Rabbit
Predicted Reactivity Dog, Cow, Pig, Guinea Pig
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TGF Beta 1
Immunogen Range 301-350/390
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7040
Swiss Prot P01137
Subcellular location Secreted, Extracellular matrix
Synonyms CED; LAP; DPD1; TGFB; TGFbeta; Transforming growth factor beta-1; TGF-beta-1; TGFB1; TGFB2; TGFB3
Background Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200