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Histone H3 (Di Methyl K36) Antibody, PerCP-Cy5.5 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Rabbit
  • Drosophila
Overview
Catalog # bs-3769R-PerCP-Cy5.5
Product Name Histone H3 (Di Methyl K36) Antibody, PerCP-Cy5.5 Conjugated
Applications FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Rabbit, Drosophila
Specifications
Conjugation PerCP-Cy5.5
Host Rabbit
Source KLH conjugated synthetic metylpeptide derived from human Histone H3 around metylation site of Di Methyl Lys36
Modification Site Di Methyl K36
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3020
Subcellular location Nucleus
Synonyms Histone H3Di Methyl Lys36; Di methyl Histone H3Lys36; Di methyl Histone H3K36; H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_TETTH; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l.
Background Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200