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FoxO3a (Ser253) Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Others

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-3140R-FITC
Product Name FoxO3a (Ser253) Polyclonal Antibody, FITC Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity This phosphorylation site is homologous to Ser252 in Mouse and Rat.
Reactivity Human, Mouse, Rat, Others
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser253
Modification Site Ser253
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2309
Swiss Prot O43524
Subcellular location Cytoplasm, Nucleus
Synonyms FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3
Background Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200