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Cyclin D1 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Sheep
  • Rabbit

Predicted Reactivity

  • Dog
Overview
Catalog # bs-0623r-pe-100ul
Product Name Cyclin D1 Polyclonal Antibody, PE Conjugated
Applications WB, FCM
Reactivity Human, Mouse, Rat, Bovine, Sheep, Rabbit
Predicted Reactivity Dog
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Cyclin D1
Immunogen Range 61-110/295
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 12443
Swiss Prot P25322
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms cD1; CycD1; Cyl-1; PRAD1; bcl-1; AI32739; G1/S-specific cyclin-D1; Ccnd1
Background Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).
Application Dilution
WB 1:300-5000
FCM 1:20-100