CD3E Polyclonal Antibody

Mouse spleen lysate probed with Anti-CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Lane 1: Human MOLT4 cell lysates; Lane 2: Human Jurkat cell lysates probed with CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human MOLT4 cell lysates; Lane 2: Human Jurkat cell lysates probed with CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IF

Reactivity

Mouse

Predicted Reactivity

Overview
Catalog #	bs-4815R
Product Name	CD3E Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IF
Reactivity	Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse CD3 epsilon
Immunogen Range	23-120/189
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	12501
Swiss Prot	P22646
Subcellular location	Cell membrane
Synonyms	CD3; T3e; AI504783; CD3epsilon; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e
Background	The CD3 complex mediates signal transduction.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF	1:100-500