BNP Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BNP Polyclonal Antibody, Unconjugated (bs-2207R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti-BNP Polyclonal Antibody, Unconjugated(bs-2207R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-BNP Polyclonal Antibody, Unconjugated(bs-2207R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Applications

ELISA
IHC-P
IF(IHC-P)

Reactivity

Predicted Reactivity

Mouse

Overview
Catalog #	bs-2207R
Product Name	BNP Polyclonal Antibody
Applications	ELISA, IHC-P, IF(IHC-P)
Reactivity	Rat
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat BNP
Immunogen Range	85-115/121
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25105
Swiss Prot	P13205
Subcellular location	Secreted
Synonyms	BNP; Bnf; Natriuretic peptides B; Gamma-brain natriuretic peptide; Iso-ANP; Nppb
Background	Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200