S100B Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-S100B Polyclonal Antibody, Unconjugated (bs-2015R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti-S100B Polyclonal Antibody, Unconjugated(bs-2015R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-S100B Polyclonal Antibody, Unconjugated(bs-2015R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human
Mouse
Cow
Chicken
Rabbit

Overview
Catalog #	bs-2015R
Product Name	S100B Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Cow, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human S100B
Immunogen Range	31-92/92
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6285
Swiss Prot	P04271
Subcellular location	Cytoplasm, Nucleus
Synonyms	NEF; S1; S1-B; S1beta; Protein S1-B; S-1 protein beta chain; S-1 protein subunit beta; S1 calcium-binding protein B; S1B
Background	Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200