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Sample Tissue\/Cells lysates probed with PPARA (7G5) Monoclonal Antibody, unconjugated (bsm-51405M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Hela cells using bsm-51405M PPARA (7G5) Antibody (green) compared to an isotype control of mouse IgG1 (blue). bsm-51405M was diluted at 1:25 dilution. An Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Mouse NIH\/3T3 cell lysates probed with PPARA Monoclonal Antibody, Unconjugated (bsm-51405M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51405M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP (C11F2) Monoclonal Antibody, Unconjugated (bsm-0391M) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Lane 1: C Reactive Protein (CRP); 40ug loaded into the lane; Probed with CRP (C11F2) Monoclonal Antibody, unconjugated (bsm-0391M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: C Reactive Protein(CRP) probed with CRP(C11F2) Monoclona Antibody, Unconjugated (bsm-0391M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U-2OS cell lysates; Lane 2: Human Siha cell lysates probed with CRP(C11F2) Monoclonal Antibody, Unconjugated bsm-0391MR) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0391M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0391M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-0391M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-0391M-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-0391M-Cy7

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-0391M-Cy5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-0391M-Cy3

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-0391M-FITC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-0391M-PE

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-0391M-BF350

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-0391M-BF405

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-0391M-BF488

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-0391M-BF555

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-0391M-BF594

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-0391M-BF647

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-0391M-BF680

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-0391M-BF750

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-0391M-APC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-0391M-APC-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-0391M-APC-Cy7

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-0391M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-0391M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-0391M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-0391M-PerCP

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-0391M-PerCP-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0391M-RBITC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC