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eIF4E Polyclonal Antibody

Image provided by the Independent Validation Program, badge number 029640:Formalin-fixed and paraffin embedded human pancreas labeled with Anti-eIF4E Polyclonal Antibody, Unconjugated (bs-4979R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Image provided by the Independent Validation Program, badge number 029640:Formalin-fixed and paraffin embedded human pancreas labeled with Anti-eIF4E Polyclonal Antibody, Unconjugated (bs-4979R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-eIF4E Polyclonal Antibody (bs-4979R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-eIF4E Polyclonal Antibody (bs-4979R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Lane 1: Mouse Colon lysates; Lane 2: Rat Colon lysates probed with eIF4E Polyclonal Antibody, Unconjugated (bs-4979R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Colon lysates; Lane 2: Rat Colon lysates probed with eIF4E Polyclonal Antibody, Unconjugated (bs-4979R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
Overview
Catalog # bs-4979R
Product Name eIF4E Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity Human, Rat
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF4E
Immunogen Range 121-217/217
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1977
Swiss Prot P06730
Subcellular location Cytoplasm
Synonyms CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Background Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

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