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CD46 (Extracellular region) Antibody

Applications

  • WB
  • IP
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70400M
Product Name CD46 (Extracellular region) Antibody
Applications WB, IP, ICC
Specificity Clone M037 mouse monoclonal antibody detects a 45-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human lung tissues. This antibody weakly detects denatured CD46. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M037) was generated from a proprietary antigen related to native human CD46 expressed in NCI-H1915 lung cancer cell line.
Clonality Monoclonal
Clone # M037
Isotype IgG2b
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P15529
Synonyms Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, CD46, MCP, MIC10
Background CD46 is a complement regulatory protein that is also called membrane cofactor protein. This protein is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by whole antiserum factor 1, thereby protecting host cells from complement-dependent cytotoxicity. CD46 can also function as a receptor for selected bacteria and viruses, and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization. CD46 is overexpressed in medulloblastoma tumors, and CD46 expression has been linked with poor prognosis in breast cancer. The upregulation of CD46 may protect cancer cells from complement-dependent cytotoxicity to facilitate cancer cell immune evasion.
Application Dilution
WB 1:300-5000
IP 1-2ug
ICC 1:100-500