New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

Axl/UFO (Extracellular region) Antibody

Applications

  • WB
  • IP
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70355M
Product Name Axl/UFO (Extracellular region) Antibody
Applications WB, IP, ICC
Specificity Clone M047 mouse monoclonal antibody detects a 140 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H1915, and MDA-MB-231 carcinomas. The antibody detects Axl in membranes and cytoplasm in MDA-MB-231 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M047) was generated from a recombinant protein that included the extracellular region of human Axl protein.
Clonality Monoclonal
Clone # M047
Isotype IgG1
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P30530
Synonyms Tyrosine-protein kinase receptor, UFO, Axl, Tyro7, ARK,
Background The Axl/UFO receptor tyrosine kinase (RTKs) family includes Axl/UFO/Tyro7, Sky/Tyro3, and c-Mer/Tyro12. These RTKs have a conserved intracellular tyrosine kinase domain and extracellular domains that include immunoglobulin-like and fibronectin-type moieties similar to those found in cell adhesion molecules. The ligand for these receptors is the vitamin Kdependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor. Upon binding to its receptor, Gas6 activates phosphatidylinositol 3- kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB. Axl is overexpressed in several cancers, including breast, lung, liver, colon, gastric, ovarian, pancreatic, and glioblastoma. The Axl/Gas6 signalling pathway has been shown to drive cancer cell survival, proliferation, migration and invasion, and several therapeutic strategies are being developed to regulate Axl cell signaling.
Application Dilution
WB 1:300-5000
IP 1-2ug
ICC 1:100-500