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ATM (C-terminal region) Antibody

Applications

  • WB
  • FCM
  • IP
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70350M
Product Name ATM (C-terminal region) Antibody
Applications WB, FCM, IP, ICC
Specificity This antibody detects a 370 kDa* protein corresponding to the molecular mass of ATM on SDS-PAGE immunoblots of human A431 and Jurkat cells.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone M361 was generated from a recombinant sequence corresponding to amino acids in the C-terminal region of human ATM.
Clonality Monoclonal
Clone # M361
Isotype IgG2b
Purification Purified by Protein A.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot Q13315
Synonyms ataxia telangiectasia mutated, AT1 ATDC TEL1 TELO1
Background Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. Activation of ATM after DNA damage involves Cdk5 mediated phosphorylation of Ser-794 followed by autophosphorylation at Ser-1891. Active ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis and DNA repair. The Cdk5–ATM pathway regulates phosphorylation and function of the ATM targets p53 and H2AX in postmitotic neurons. Other known substrates of ATM include Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1. Thus, activation of Cdk5 by DNA damage may be an important initiator of ATM-dependent regulation of cell cycle checkpoints.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IP 1-2ug
ICC 1:100-500