ATG10 Recombinant Monoclonal Antibody

$Western blot analysis of HeLa cell lysate. Using ATG10 (bsm-61475R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HeLa cell lysate. Using ATG10 (bsm-61475R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human

Overview
Catalog #	bsm-61475r-100ul
Product Name	ATG10 Recombinant Monoclonal Antibody
Applications	WB, IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATG10
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	83734
Swiss Prot	Q9H0Y0
Subcellular location	Cytoplasm
Synonyms	APG10L;ATG10 autophagy related 10 homolog (S. cerevisiae); ATG10 autophagy related 10 homolog; Autophagy related protein 10.
Background	E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3.
Application Dilution
WB	1:300-5000
IP	1-2ug