AKT1S1 Recombinant Monoclonal Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate. Using AKT1S1 (bsm-61150R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate. Using AKT1S1 (bsm-61150R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61150r-100ul
Product Name	AKT1S1 Recombinant Monoclonal Antibody
Applications	WB, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AKT1S1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84335
Swiss Prot	Q96B36
Subcellular location	Cytoplasm
Synonyms	40 kDa proline rich AKT substrate; AKT1 S1; Lobe; PRAS40; Proline rich akt substrate; Proline rich Akt substrate 40 kDa.
Background	Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
Application Dilution
WB	1:300-5000
IP	1-2ug