AKT1S1 Recombinant Monoclonal Antibody

$Western blot analysis of 293T cell lysate. Using AKT1S1 (bsm-61149R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of 293T cell lysate. Using AKT1S1 (bsm-61149R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IHC-P
IHC-F
IP
IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-61149r-100ul
Product Name	AKT1S1 Recombinant Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IP, IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AKT1S1
Clonality	Monoclonal
Clone #	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84335
Swiss Prot	Q96B36
Subcellular location	Cytoplasm
Synonyms	40 kDa proline rich AKT substrate; AKT1 S1; AKT1 substrate 1 (proline rich); AKT1S1; Lobe; PRAS40; Proline rich akt substrate.
Background	Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF