ABCA1 monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: Mouse liver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 180 S\r\nPredicted MW: 254 kDa\r\nObserved MW: 254 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: Mouse liver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 180 S\r\nPredicted MW: 254 kDa\r\nObserved MW: 254 kDa

Applications

Reactivity

Mouse

Predicted Reactivity

Human

Overview
Catalog #	bsm-60239M
Product Name	ABCA1 monoclonal Antibody
Applications	WB, IF, ICC
Reactivity	Mouse
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human ABCA1
Clonality	Monoclonal
Clone #	H5D5
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	19
Swiss Prot	O95477
Subcellular location	Cytoplasm
Synonyms	ABC1; ATP binding cassette transporter A1; ABC 1; ABC Transporter 1; ABCA 1; ATP binding Cassette 1; ATP binding cassette sub family A ABC1 member 1; ATP binding cassette sub family A member 1; ATP binding cassette sub-family A member 1; ATP binding Cassette Transporter 1; ATP-binding Cassette 1; ATP-binding Cassette Transporter 1; CERP; Cholesterol Efflux Regulatory Protein; FLJ14958; HDLDT1; Membrane bound; MGC164864; MGC165011; TD; TGD; ABCA1_HUMAN.
Background	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency. [provided by RefSeq, Sep 2019]
Application Dilution
WB	=1:500-2000
IF	ICC=1:100-500
ICC	IF=1:100-500