Asymmetric Di-Methyl-Histone H4 (Arg3) Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa

Applications

Reactivity

Human
Mouse
Rat
Monkey

Overview
Catalog #	bsm-60142M
Product Name	Asymmetric Di-Methyl-Histone H4 (Arg3) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat, Monkey
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	D5C7
Isotype	IgG
Concentration	1mg/ml
Purification	Protein G coupled with the antigen peptide containing H4R3me2 (asy).
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P62805
Application Dilution
WB	1:300-5000