ATP2A1 (3G1) Monoclonal Antibody

$Lane 1: Human Fetal Skeletal Muscle; Probed with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Fetal Skeletal Muscle; Probed with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.$

Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ATP2A1 (3G1) Monoclonal Antibody (bsm-54466R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Applications

WB
IHC-P
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-54466R
Product Name	ATP2A1 (3G1) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human SERCA1 ATPase aa 1-50.
Clonality	Monoclonal
Clone #	3G1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	487
Swiss Prot	O14983
Subcellular location	Membrane bound, Endoplasmic reticulum
Synonyms	Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; ATP2A1; SERCA1; SR Ca(2+)-ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Background	Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IP	1-2ug