5HT2C Receptor (4C3) Monoclonal Antibody

$Lane 1: A431 Cells; Lane 2: K562 Cells; Probed with 5HT2C Receptor (4C3) Monoclonal Antibody (bsm-54055R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n$

Lane 1: A431 Cells; Lane 2: K562 Cells; Probed with 5HT2C Receptor (4C3) Monoclonal Antibody (bsm-54055R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with 5HT2C Receptor (4C3) Monoclonal Antibody (bsm-54055R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with 5HT2C Receptor (4C3) Monoclonal Antibody (bsm-54055R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54055R
Product Name	5HT2C Receptor (4C3) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C-terminal human 5HT2C Receptor
Clonality	Monoclonal
Clone #	4C3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3358
Swiss Prot	P28335
Synonyms	5-hydroxytryptamine receptor 2C, 5-hydroxytryptamine receptor 1C, Serotonin receptor 2C, 5-HT-2C, 5-HT2C, 5-HTR2C, 5-HT-1C, 5-HT1C, HTR2C, HTR1C
Background	G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400