ABCF1 (4B12) Monoclonal Antibody

$Hela cells lysate probed with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Hela cells lysate probed with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:300 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:300 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat stomach tissue incubated with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat stomach tissue incubated with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with ABCF1 (4B12) Monoclonal Antibody (bsm-52620R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Rat

Overview
Catalog #	bsm-52620R
Product Name	ABCF1 (4B12) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein of human ABCF1 between 780 to 840 amino acids
Clonality	Monoclonal
Clone #	4B12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	23
Swiss Prot	Q8NE71
Synonyms	ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein, ABCF1
Background	Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC