Adiponectin receptor protein 1 (5G11) Monoclonal Antibody

Flow cytometric analysis of HepG2 cells with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Human skeletal muscle lysate probed with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Human skeletal muscle lysate probed with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in LO2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in LO2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52557R
Product Name	Adiponectin receptor protein 1 (5G11) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein of human Adiponectin Receptor 1 corresponding to aa 1-100
Clonality	Monoclonal
Clone #	5G11
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	51094
Swiss Prot	Q96A54
Synonyms	Adiponectin receptor protein 1, Progestin and adipoQ receptor family member I, ADIPOR1
Background	Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC