Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody

$Mouse kidney Probed with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody (bsm-52486R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Mouse kidney Probed with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody (bsm-52486R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody (bsm-52486R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody (bsm-52486R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody, Unconjugated (bsm-52486R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody, Unconjugated (bsm-52486R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody, Unconjugated (bsm-52486R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody, Unconjugated (bsm-52486R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Mouse Brain Lysates; Lane 2: Human K562 cell Lysates. Probed with Acetyl CoA Carboxylase 1(ACC1) monoclonal Antibody, unconjugated (bsm-52486R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Brain Lysates; Lane 2: Human K562 cell Lysates. Probed with Acetyl CoA Carboxylase 1(ACC1) monoclonal Antibody, unconjugated (bsm-52486R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52486R
Product Name	Acetyl CoA Carboxylase 1(ACC1) (2D3) Monoclonal Antibody
Applications	WB, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human ACC1 between 350 to 450 amino acids
Clonality	Monoclonal
Clone #	2D3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	32
Swiss Prot	O00763
Synonyms	Acetyl-CoA carboxylase 2, ACC-beta, ACACB, ACC2, ACCB
Background	Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Involved in inhibition of fatty acid and glucose oxidation and enhancement of fat storage (By similarity). May play a role in regulation of mitochondrial fatty acid oxidation through malonyl-CoA-dependent inhibition of carnitine palmitoyltransferase 1 (By similarity).
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IHC