5T4 (23B1) Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF-7, 2: JAR\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 46 kDa\r\nObserved MW: 70-80\/85 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF-7, 2: JAR\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 46 kDa\r\nObserved MW: 70-80\/85 kDa

$Tissue: Human placenta\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-52422R$

Tissue: Human placenta\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-52422R

$IP of MCF-7 cells extracts\r\nIP ab incubation condition: PTM-6392, 4\u00b0C \r\novernight, 1:100 dilution\r\nWB primary ab incubation condition: bsm-52422R, room temperature 2h, 1:1000 dilution\r\nSecondary ab: Anti-Rabbit IgG for IP (HRP)\r\nBlocking buffer and concentration: 5%\r\nNFDM\/TBST\r\nDiluting buffer and concentration: 5%\r\nNFDM\/TBST\r\n1: 5% Input\r\n2: IP with bsm-52422R\r\n3: IP with Rabbit monoclonal IgG Isotype\r\nObserved MW: 70-80\/85 kDa\r\nExposure time: 120 s$

IP of MCF-7 cells extracts\r\nIP ab incubation condition: PTM-6392, 4\u00b0C \r\novernight, 1:100 dilution\r\nWB primary ab incubation condition: bsm-52422R, room temperature 2h, 1:1000 dilution\r\nSecondary ab: Anti-Rabbit IgG for IP (HRP)\r\nBlocking buffer and concentration: 5%\r\nNFDM\/TBST\r\nDiluting buffer and concentration: 5%\r\nNFDM\/TBST\r\n1: 5% Input\r\n2: IP with bsm-52422R\r\n3: IP with Rabbit monoclonal IgG Isotype\r\nObserved MW: 70-80\/85 kDa\r\nExposure time: 120 s

Applications

WB
IHC-P
IP

Reactivity

Human

Overview
Catalog #	bsm-52422R
Product Name	5T4 (23B1) Monoclonal Antibody
Applications	WB, IHC-P, IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human 5T4 corresponding to aa 80-240
Clonality	Monoclonal
Clone #	23B1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7162
Swiss Prot	Q13641
Synonyms	Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, M6P1, Wnt-activated inhibitory factor 1, 5T4 oncotrophoblast glycoprotein, WAIF1, TPBG
Background	May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Application Dilution
WB	1:500-1000
IHC-P	1:1000-2000
IP	1:50-100