CD10 Monoclonal Antibody

$Lane 1: CEM cell lysates probed with CD10 Monoclonal Antibody, Unconjugated (bsm-51496M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: CEM cell lysates probed with CD10 Monoclonal Antibody, Unconjugated (bsm-51496M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human

Overview
Catalog #	bsm-51496M
Product Name	CD10 Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human CD10
Immunogen Range	225-300/750
Clonality	Monoclonal
Clone #	B12H1
Isotype	IgM
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4311
Swiss Prot	P08473
Subcellular location	Cell membrane
Synonyms	MME; NEP; NEP_HUMAN; Atriopeptidase; CALLA; CD 10; CD10; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; gp100; Membrane metallo endopeptidase; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; Neprilysin; Neutral endopeptidase 24.11; Neutral endopeptidase; Neprilysin; Skin fibroblast elastase; SFE.
Background	Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Application Dilution
WB	=1:100-1000Ôºå