ATG4C (7C2) Monoclonal Antibody

$Mouse heart lysates; probed with ATG4C (7C2) Monoclonal Antibody, unconjugated (bsm-51084M) overnight at 4\u00b0C followed by a conjugated secondary antibody.$

Mouse heart lysates; probed with ATG4C (7C2) Monoclonal Antibody, unconjugated (bsm-51084M) overnight at 4\u00b0C followed by a conjugated secondary antibody.

Applications

Reactivity

Mouse

Overview
Catalog #	bsm-51084M
Product Name	ATG4C (7C2) Monoclonal Antibody
Applications	WB
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This ATG4C monoclonal antibody is generated from mouse immunized with ATG4C recombinant protein.
Clonality	Monoclonal
Clone #	7C2
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	84938
Swiss Prot	Q96DT6
Synonyms	APG4C; AUTL1; AUTL3; APG4-C; Cysteine protease ATG4C; AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; ATG4C
Background	Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Application Dilution
WB	1:300-5000