ANO1/TMEM16A Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P

Reactivity

Human

Overview
Catalog #	bsm-49061M
Product Name	ANO1/TMEM16A Monoclonal Antibody
Applications	IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human ANO1/TMEM16A
Clonality	Monoclonal
Isotype	IgG1
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55107
Swiss Prot	Q5XXA6
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ANO 1;ANO1_HUMAN; Anoctamin 1; Anoctamin 1 calcium activated chloride channel; Anoctamin-1; Anoctamin1; Discovered on gastrointestinal stromal tumors protein 1; DOG 1; FLJ10261; Membrane protein; Oral cancer overexpressed 2; Oral cancer overexpressed protein 2; ORAOV 2; ORAOV2; TAOS 2; TAOS2; TMEM 16A; TMEM16A; Transmembrane protein 16A (eight membrane spanning domains); Transmembrane protein 16A; Tumor amplified and overexpressed sequence 2; Tumor-amplified and overexpressed sequence 2.
Background	Acts as a calcium-activated chloride channel. Required for normal tracheal development (By similarity).
Application Dilution
IHC-P	1:200-400