Overview |
bsm-33166M-BF647 |
Strep-Tag II (Mix-mA™) Monoclonal Antibody, AbBy Fluor® 647 Conjugated |
WB |
Tags, Others |
Specifications |
AbBy Fluor® 647 |
Mouse |
KLH conjugated synthetic peptide of Strep-Tag |
Monoclonal |
Mix-mA™ |
IgG |
1ug/ul |
Purified by Protein A. |
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
Target |
Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep-Tag-II. |
The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. |
Application Dilution |
WB |
1:300-5000 |