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5 MethylCytosine Polyclonal Antibody

Hela cells fixed with 4% paraformaldehyde; permeabilize with ice cold methanol; blocking with 1 X PBS containing 5% BSA; probed with 5 Methyl Cytosine Polyclonal Antibody, Unconjugated (bs-9450R) (green) at 1:50 for 30 minutes followed by a PE conjugated secondary antibody compared to isotype control (orange).
Hela cells fixed with 4% paraformaldehyde; permeabilize with ice cold methanol; blocking with 1 X PBS containing 5% BSA; probed with 5 Methyl Cytosine Polyclonal Antibody, Unconjugated (bs-9450R) (green) at 1:50 for 30 minutes followed by a PE conjugated secondary antibody compared to isotype control (orange).
Raji Cells fixed with 4% paraformaldehyde; permeabilize with ice cold methanol; blocking with 1 X PBS containing 5% BSA; probed with 5 Methyl Cytosine Polyclonal Antibody, Unconjugated (bs- 9450R) (green) at 1:33 for 30 minutes followed by a PE conjugated secondary antibody compared to isotype control (orange).
Raji Cells fixed with 4% paraformaldehyde; permeabilize with ice cold methanol; blocking with 1 X PBS containing 5% BSA; probed with 5 Methyl Cytosine Polyclonal Antibody, Unconjugated (bs- 9450R) (green) at 1:33 for 30 minutes followed by a PE conjugated secondary antibody compared to isotype control (orange).

Applications

  • WB
  • FCM
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Human
  • Others
Overview
Catalog # bs-9450R
Product Name 5 MethylCytosine Polyclonal Antibody
Applications WB, FCM, IHC-P, IF(IHC-P)
Reactivity Human, Others
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated 5 MethylCytosine
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms 5-Methyl Cytidine; 5 m C; 5 mC; 5 me C; 5 Me Cytidine; 5 MeCyd; 5 Methyl Cytidine; 5 MethylCytosine; 5-mC; 5-Me Cytidine; 5-Methyl-Cytosine; 5-MethylCytidine.
Background Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

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