Connexin 35/36 (Ser276) Antibody

Applications

Reactivity

Mouse
Rabbit
Fish

Overview
Catalog #	bs-70049r-100ul
Product Name	Connexin 35/36 (Ser276) Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~35 kDa connexin-35/36 protein phosphorylated at Ser276.
Reactivity	Mouse, Rabbit, Fish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues of perch connexin 35/36 surrounding Ser276, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser276
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot	O93328
Synonyms	Connexin 36 / GJA9 antibody, Connexin 36 antibody, Connexin-36 antibody, Cx36 antibody, CXD2_HUMAN antibody, Gap junction alpha-9 protein antibody, Gap junction delta-2 protein antibody, Gap junction protein alpha 9 antibody, gap junction protein, alpha 9, 36kDa antibody, JA9 antibody, GJD2 antibody, MGC138315 antibody, MGC138319 antibody, Connexin 35 antibody, Connexin-35 antibody, Cx35 antibody, Cx35.4 antibody
Background	Connexin 35/36 is the most abundant gap junction protein in the central nervous system. Connexin gap junction coupling in the retina has been shown to be regulated by light exposure and neurotransmitter signaling, but more recently has been observed to also depend on direct phosphorylation of connexin (Ouyang et al., 2005, Patel et al., 2006). PKA phosphorylation of Ser110 in the intracellular loop and Ser276 in the C-terminal domain can cause uncoupling of connexin 35 gap junctions (Ouyang et al., 2005). The largest uncoupling effect is seen when both of these sites are phosphorylated, with a much smaller degree of uncoupling observed when only one is phosphorylated (Kothmann et al., 2007). C-terminal domain phosphorylation of connexin 35 at Ser276 is observed in teleost, amphibian, and avian species, with connexin 36 phosphorylation at Ser293 being the mammalian equivalent.
Application Dilution
WB	1:300-5000
IHC