Aquaporin 2 (Ser269) Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Bovine

Overview
Catalog #	bs-70019r-100ul
Product Name	Aquaporin 2 (Ser269) Antibody
Applications	WB, IP, IHC
Specificity	Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser269. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Bovine
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 of rat aquaporin 2, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser269
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	25386
Swiss Prot	P34080
Synonyms	ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody
Background	Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al., 2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Serine-269 has been recently identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).
Application Dilution
WB	1:300-5000
IP	1-2ug
IHC