ML323 --- USP1-UAF1 Inhibitor

Overview
Catalog #	bs-60188c-2-mg-solid
Product Name	ML323 --- USP1-UAF1 Inhibitor
Specifications
Storage Buffer	Powder
Storage Condition	Store in dry, dark place at -20C for 1 year.
Target
Product Information	Molecular Weight: 384.48 Formula: C23 H24 N6 CAS Number: 1572414-83-5 InChi Key: VUIRVWPJNKZOSS-UHFFFAOYSA-N InChi: InChI=1S/C23H24N6/c1-16(2)20-6-4-5-7-21(20)23-24-14-17(3)22(27-23)25-15-18-8-10-19(11-9-18)29-13-12-26-28-29/h4-14,16H,15H2,1-3H3,(H,24,25,27) Smiles: CC1=CN=C(N=C1NCC1C=CC(=CC=1)N1C=CN=N1)C1C=CC=CC=1C(C)C Purity: 98.0 Solubility: DMSO up to 50 mM Appearance: Solid Power. Shelf Life: 1.0 years
Description	ML323 is a potent, selective and reversible inhibitor of the USP1-UAF1 deubiquitinase complex with IC50 ~76 nM in the Ub-Rho assay. It has excellent selectivity against human DUBs, deSUMOylase, deneddylase and unrelated proteases, showed little to no inhibition against other USPs tested, including USP2, USP5, USP7, USP8, USP10, USP11, USP14 and USP21. Inhibition mechanism and HDX studies suggest that ML323 binds away from the USP1 active site. ML323 inhibits the deubiquitination of PCNA and FANCD2. Inhibition of USP1–UAF1 by ML323 impairs homologous recombination and sister chromatid exchange in U2OS cells. Moreover, ML323 potentiates cisplatin cytotoxicity in non-small cell lung cancer and osteosarcoma cells. A notable feature of ML323 is that it simultaneously targets two major DNA damage response pathways (TLS and FA) by inhibiting a common deubiquitinase. USP1-UAF1 could be a key regulator of the DNA damage response and a target for overcoming resistance to the platinum-based anticancer drugs.