ENPP7 Polyclonal Antibody

$FHC Cell lysates probed with ENPP7 Polyclonal Antibody, unconjugated (bs-4277R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

FHC Cell lysates probed with ENPP7 Polyclonal Antibody, unconjugated (bs-4277R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$HT29 Cell lysates probed with ENPP7 Polyclonal Antibody, unconjugated (bs-4277R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

HT29 Cell lysates probed with ENPP7 Polyclonal Antibody, unconjugated (bs-4277R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ENPP7 Polyclonal Antibody, Unconjugated (bs-4277R), followed by polypeptide blocking at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ENPP7 Polyclonal Antibody, Unconjugated (bs-4277R), followed by polypeptide blocking at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ENPP7 Polyclonal Antibody, Unconjugated (bs-4277R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ENPP7 Polyclonal Antibody, Unconjugated (bs-4277R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Rabbit

Overview
Catalog #	bs-4277R
Product Name	ENPP7 Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ENPP7
Immunogen Range	351-458/458
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	339221
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ALK SMase; Alk-SMase; Alkaline sphingomyelin phosphodiesterase; E-NPP 7; Ectonucleotide pyrophosphatase/phosphodiesterase 7; Ectonucleotide pyrophosphatase/phosphodiesterase family member 7; ENPP7; ENPP7_HUMAN; Intestinal alkaline sphingomyelinase; NPP-7.
Background	Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400