CSFV E2 Protein Polyclonal Antibody

$Lane 1: Recombinant CSFV E2 protein probed with CSFV E2 Polyclonal Antibody, Unconjugated (bs-20116G) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant CSFV E2 protein probed with CSFV E2 Polyclonal Antibody, Unconjugated (bs-20116G) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Virus

Overview
Catalog #	bs-20116G
Product Name	CSFV E2 Protein Polyclonal Antibody
Applications	WB, ELISA, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Virus
Specifications
Conjugation	Unconjugated
Host	Goat
Source	Recombinded CSFV E2 protein; classical swine fever virus (strain Alfort) (CSFV) (Hog cholera virus)
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P19712
Synonyms	CSFV Polyprotein; CSFV E2 protein; CSFV E2; polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; CSFV Envelope glycoprotein E2; Classical swine fever virus E2; Classical swine fever virus Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus].
Background	E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane. P7 forms a leader sequence to properly orient NS2 in the membrane. Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200