AMPK alpha-1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-AMPK alpha-1\/PRKAA1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-AMPK alpha-1\/PRKAA1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded mouse colon labeled with Anti-AMPK alpha-1\/PRKAA1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-AMPK alpha-1\/PRKAA1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human kidney labeled with Anti-AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Human Hela lysates probed with AMPK alpha 1 Polyclonal Antibody, Unconjugated (bs-1115R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Mouse spleen cells probed with AMPK alpha-1 Polyclonal Antibody, unconjugated (bs-1115R) at 1:100 dilution for 30 minutes compared to control cells (purple), secondary only (blue) and isotype control (orange)

U937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AMPK alpha-1 Polyclonal Antibody(bs-1115R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$A549 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at -20\u2103 for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AMPK alpha 1) polyclonal Antibody, Unconjugated (bs-1115R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

A549 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at -20\u2103 for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AMPK alpha 1) polyclonal Antibody, Unconjugated (bs-1115R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AMPK alpha 1 Polyclonal Antibody(bs-1115R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AMPK alpha 1 Polyclonal Antibody(bs-1115R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
FCM
IHC-P
IHC-F

Reactivity

Human
Mouse
Rat
Pig
Sheep

Predicted Reactivity

Dog
Cow
Horse
Chicken

Overview
Catalog #	bs-1115R
Product Name	AMPK alpha-1 Polyclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F
Reactivity	Human, Mouse, Rat, Pig, Sheep
Predicted Reactivity	Dog, Cow, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AMPK alpha 1
Immunogen Range	351-450/559
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5562
Swiss Prot	Q13131
Subcellular location	Cytoplasm, Nucleus
Synonyms	AMPK; AMPKa1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA1; AMPK1
Background	The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500